Historical Background on Gamete and Embryo Cryopreservation.- Utility of Animal Models for Human Ovarian Tissue Cryopreservation.- Current Challenges in Immature Oocyte Cryopreservation.- Role of Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification.- Slow Freezing of Human Sperm.- Technology of Aseptic Cryoprotectant-free Vitrification of Human ICSI Spermatozoa.- Human Epididymal and Testicular Sperm Cryopreservation.- Human Oocytes Slow-rate Freezing: Methodology.- Slow Freezing and Thawing of Human Cleavage Stage Embryos.- Human Oocyte Vitrification.- Human Embryo Vitrification.- Human Ovarian Tissue Slow Freezing.- Human Ovarian Tissue Vitrification.- Establishing an Oocyte Cryo Bank Network.- Development of a Nationwide Network for Ovarian Tissue Crypreservation.- Directional Freezing of Ovarian Tissue and Freeze-drying of Stem Cells for Fertility Preservation.- Sterile Plate-BasedVitrification of Adherent Human Pluripotent Stem Cells and Their Derivatives Using the TWIST Method.- Vitrification: A Reliable Method for Cryopreservation of Animal Embryos.- Cryopreservation Effect on Genetic Function: Neonatal Outcomes.- Gavi-Automated Vitrification Instrument.- A. Cryotec® Vitrification Thawing.- B. Solid Surface Vitrification.- C. Automated Vitrification of Mammalian Embryos on a Digital Microfluidic Device.- D. Irvine Scientific® Vitrification System.- E. Rapid-i: Closed Vitrification Device by Vitrolife.- F. Quinn’s Advantage Embryo Freeze Kit.- G. Vitrification of Blastocysts Using VitriBlast™ and ThermoBlast™ – Nidacon.
Dr. Ashok Agarwal is the Head of the Clinical Andrology
Center, which over the years under his leadership, has become a
center of excellence in the United States for the diagnosis of male
infertility and fertility preservation of men with oncological
conditions. He is board certified as a Clinical Laboratory Director
in Andrology by the American Board of Bioanalysis and an Inspector
for the College of American Pathologists “Reproductive Laboratory
Program” for accreditation of Andrology & IVF Laboratories. Dr.
Agarwal is the recipient of several awards, such as the 2011
Innovator Award from Cleveland Clinic Innovations for the
development of “Remote Sperm Banking Kits.” His current research
interests are identifying biological markers of oxidative stress,
DNA damage, and apoptosis using proteomic research tools and
bioinformatics analysis, as well as preserving fertility in
patients with cancer.
Zsolt Peter Nagy, M.D.,
Ph.D., is the Scientific Director at Reproductive Biology
Associates (RBA), in Atlanta, GA, USA. He has acquired distinctive
knowledge and experience on embryo science. He is well known for
his contributions to oocyte vitrification and the development of
the largest donor oocyte cryo-bank in North America. Dr. Nagy is a
member/officer of several national and international professional
societies, including ASRM, ESHRE, Alpha, ISFP and AFP, as well as a
reviewer and editor. He is the author and/or co-author of over 200
publications, book chapters, and books.
Dr. Alex C.
Varghese is the Scientific and Laboratory Director at Astra
Fertility group in Mississauga, Canada. His proficiency and vast
experience in clinical embryology and academics earned him a
reputation in the ART community. He is well regarded for his
passion to disseminate knowledge through online platforms and also
as a leader of embryology society. He has helped many units
establish training programs and affordable batch IVF units in many
South Asian Countries. His area of interest is in developing
automated systems for assisted reproduction. Dr. Varghese believes
in “responsible reproduction” through ethical practices, low-cost
IVF for the masses, quality management, and safe practices that
safe guard the health of IVF children.
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