Table of Contents

SECTION I: LABORATORY INSIGHTS The Biology of Fertilization in Humans 3 Laura Hewitson and Gerald P. Schatten The New Imaging Technique Applied to Human Reproduction 17 Cathy Boutin and Clifford Hoyt The Oocyte 25 Vanessa Guelman and Pasquale Patrizio Fertilization and Cleavage 49 Vanessa Guelman Classification of Pronuclei and Polarity of the Zygote: Correlations with Outcome 71 Lynette Scott Intracytoplasmic Sperm Injection 89 Vanessa Guelman and Michael J. Tucker Morphological Scoring of Human Embryos and Its Relevance to Blastocyst Transfer 99 Michael J. Tucker and Juergen Liebermann Assisted Hatching 109 Marc P. Portmann and Ronald F. Feinberg Oocyte and Embryo Cryopreservation 137 Michael J. Tucker and Juergen Liebermann Practical Aspects of Human Ovarian Tissue Storage 161 Debra A. Gook and David H. Edgar Polar Body Biopsy, Embryo Biopsy, and Fixation for Fluorescent In Situ Hybridization Analysis 179 Santiago Munne, Mireia Sandalinas, Esther Velilla, and Peter Nagy Nuclear and Cytoplasmic Transfer in Mouse and Human Oocytes 195 John Zhang, Hui Liu, Lewis Krey, and Jamie A. Grifo SECTION II: CLINICAL INSIGHTS The Clinical Component of Assisted Reproductive Technologies 209 Pasquale Patrizio The Technique of Ovarian Transplantation: Laboratory and Clinical Aspects 229 Kutluk Oktay and Erkan Buyuk SECTION III: ADDITIONAL TOPICS Media Used in the Assisted Reproductive Technologies Laboratories 241 Patrick Quinn Cryopreservation Protocols 257 Michael J. Tucker, Paula C. Morton, and Juergen Liebermann Quality Control and Accreditation 277 Brooks A. Keel and Tammie K. Schalue The Ethics of Stem Cell Therapy 297 Glenn McGee, Pasquale Patrizio, Vanessa Kuhn, and Claire Robertson-Kraft Subject Index 311