Table of Contents

I. Fundamentals of biotechnology.- Opportunities for biotechnology in transfusion medicine.- to molecular genetics and recombinant DNA technology.- Molecular genetic analysis of HLA class II polymorphism.- Bioengineering in blood transfusion medicine.- Discussion.- II. Laboratory application of biotechnology.- Biotechnology in glood group serology — Application and trends for the future.- The characterization of monoclonal antibodies directed against carbohydrate epitopes by immunochemical means; the importance of such monoclonal antibodies for the recognition of blood group antigens.- Biochemistry of HLA-B27 in transfected cells and transgenic mice.- Biotechnology in the development of HIV diagnostic procedures.- Biotechnology and the development of HBV diagnostic procedures.- Monoclonal antibodies in blood coagulation.- Discussion.- III. Biotechnology and blood components.- Monoclonal antibodies in the production of purified proteins from human plasma.- Production of clotting factors through biotechnology.- Mammalian cell expression and characterization of recombinant human antitrhombin III.- Production and testing of rDNA hepatitis B vaccine.- Production and application of monoclonal antibodies in T-cell depletion of bone marrow for transplantation.- LAK cell production: Application of rDNA and cell culture technology.- Biotechnology of hemopoietic cells in culture.- Discussion.- IV. Clinical application and future directions.- Use of gene probes for carrier detection and prenatal diagnosis in hemophillia A.- In vivo characteristics of rDNA Factor VIII: The impact for the future in hemophilia care.- Clinical experience with monoclate®, a monoclonal antibody purified Factor VIII preparation from human plasma.- Depletion of lymphocytes from bone marrow —indications, expectations and clinical experience.- Hematopoietic hormones in transfusion medicine.- Discussion.